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| Registros recuperados : 33 | |
| 3. |  | BATISTA, J. S. S.; RODRIGUES, E. P.; HUNGRIA, M. Extração de proteínas de raízes de soja para análise proteômica. In: CONGRESSO BRASILEIRO DE SOJA, 5.; MERCOSOJA 2009, Goiânia. Anais... Londrina: Embrapa Soja, 2009. Seção trabalhos, t. 172. 1 CD-ROM. Editado por Adilson de Oliveira Júnior, Odilon Ferreira Saraiva, Clara Beatriz Hoffmann Campo, César de Castro.| Biblioteca(s): Embrapa Soja. |
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| 5. | | SCHIAVON, A. L.; RODRIGUES, E. P.; BATISTA, J. S. S.; GOMES, D. F.; HUNGRIA, M. Análise proteômica de raízes de feijão (Phaseolus vulgaris) inoculadas com Rhizobium tropici. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 5., 2010, Londrina. Resumos... Londrina: Embrapa Soja, 2010. p. 9-12. (Embrapa Soja. Documentos, 323). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo.| Biblioteca(s): Embrapa Soja. |
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| 6. | | OLIVEIRA, L. R.; MARCELINO, F. C.; BARCELLOS, F. G.; RODRIGUES, E. P.; MEGIAS, M.; HUNGRIA, M. The nodC, nodG, and glgX genes of Rhizobium tropici strain PRF 81. Functional & Integrative Genomics, Heidelberg, v. 10, n. 3, p. 425-431, Aug. 2010.| Biblioteca(s): Embrapa Soja. |
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| 7. | | BATISTA, J. S. da S.; RODRIGUES, E. P.; TORRES, A. R.; GOMES, D. F.; HUNGRIA, M. Efeito da inoculação com Bradyrhizobium japonicum no proteoma de raízes de soja. In: CONGRESSO BRASILEIRO DE MICROBILOGIA, 26.; SIMPÓSIO INTERNACIONAL DE BACTÉRIAS LÁTICAS; ENCONTRO NACIONAL DE PROFESSORES DE MICROBIOLOGIA; SIMPÓSIO DE COLEÇÕES DE CULTURAS, 4., 2011, Foz do Iguaçu. Anais... São Paulo: SBM, 2011. Resumo, 776-1.| Biblioteca(s): Embrapa Soja. |
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| 9. | | OLIVEIRA, L. R.; RODRIGUES, E. P.; MARCELINO-GUIMARÃES, F. C.; OLIVEIRA, A. L. M.; HUNGRIA, M. Fast induction of biosynthetic polysaccharide genes lpxA, lpxE, and rkpI of Rhizobium sp. strain PRF 81 by common bean seed exudates is indicative of a key role in symbiosis. Functional & Integrative Genomics, v. 13, n. 2, p. 275-283, Jun. 2013.| Biblioteca(s): Embrapa Soja. |
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| 10. | | RODRIGUES, E. P.; BATISTA, J. S. S.; TORRES, A. R.; HUNGRIA, M. Extração de proteínas de raízes de soja para análise proteômica. In: CONGRESSO BRASILEIRO DE SOJA, 5.; MERCOSOJA 2009, Goiânia. Resumos... Londrina: Embrapa Soja, 2009. p. 102, trab. 172. Editado por Adilson de Oliveira Júnior, Odilon Ferreira Saraiva, Clara Beatriz Hoffmann Campo, César de Castro.| Biblioteca(s): Embrapa Soja. |
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| 11. | | BARCELLOS, F. G.; ARAÚJO, T. G. F. de; RODRIGUES, E. P.; HUNGRIA, M. Taxonomic identification and molecular phylogeny of rhizobia isolates that nodulate bean from Paraná soils. In: BRAZILIAN CONGRESS OF GENETICS, 66.; SIMPÓSIO DE CITOGENÉTICA E GENÉTICA DE PEIXES, 19.; REUNIÃO DE GENÉTICA DE MICRORGANISMOS, 32.; CONGRESSO LATINO-AMERICANO DE GENÉTICA PARA CONSERVAÇÃO, 2., 2021, online. Abstracts... Ribeirão Preto: SBG, 2021. e-book. p. 365.| Biblioteca(s): Embrapa Soja. |
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| 12. | | RODRIGUES, E. P.; TORRES, A. R.; BATISTA, J. S. da S. B.; HUERGO, L.; HUNGRIA, M. A simple, economical and reproducible protein extraction protocol for proteomics studies of soybean roots. Genetics and Molecular Biology, Ribeirão Preto, v. 35, n. 1, suppl., p. 348-352, May 2012.| Biblioteca(s): Embrapa Soja. |
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| 14. | | RODRIGUES, E. P.; COELHO, M. S.; SOARES, C. de P.; VIDAL, M. S.; ARAÚJO, J. L. S.; BALDANI, J. I. Identificação e quantificação de compostos indólicos no sobrenadante de Gluconacetobacter dizaotrophicus, cultivada em meio liquido, por cromatografia líquida de alta eficiência (HPLC). Seropédica: Embrapa Agrobiologia, 2009. 25 p. (Embrapa Agrobiologia. Boletim de pequisa e desenvolvimento, 52).| Biblioteca(s): Embrapa Unidades Centrais. |
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| 16. | | RODRIGUES, E. P.; SOARES, C. P.; OLIVEIRA, A. L. M.; VIDAL, M. S.; SIMÕES-ARAÚJO, J. L.; BALDANI, J. I. Mutantes de Gluconacetobacter diazotrophicus (estirpe PAL5) no gene que codifica um amino oxidase são deficientes na biossíntese de ácido indol-3-acético. In: CONGRESSO BRASILEIRO DE GENÉTICA, 53., 2007, Águas de Lindóia, SP. Resumos... Águas de Lindóia: SBG, 2007. 1 p. 1 CD-ROM.| Biblioteca(s): Embrapa Agrobiologia. |
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| 17. | | GALVÃO, P. Q.; RODRIGUES, E. P.; ROUWS, L. F. M.; ARAUJO, J. L. S. de; VIDAL, M. S.; BALDANI, J. I. Analysis of Gluconacetobacter diazotrophicus PAL5 nitB mutant. In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN WITH NON-LEGUMES, 12.; INTERNATIONAL INCT SYMPOSIUM ON NITROGEN BIOLOGICAL FIXATION, 2., 03 a 8 de outubro, 2010, Búzios, RJ. Book of abstracts... Seropédica: Embrapa Agrobiologia, 2010. SI 13| Biblioteca(s): Embrapa Agrobiologia. |
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| 19. | | GALVÃO, P. G.; RODRIGUES, E. P.; ROUWS, L. F. M.; ARAUJO, J. L. S. de; VIDAL, M. S.; BALDANI, J. I. Geração e avaliação de um mutante nitB de Gluconacetobacter diazotrophicus. In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 10., 18 a 24 de outubro de 2010, Seropédica. Ciência para o Desenvolvimento Sustentável: anais... Seropédica: Embrapa Agrobiologia, 2010.| Biblioteca(s): Embrapa Agrobiologia. |
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| 20. | | RODRIGUES, E. P.; COELHO, M. S.; SOARES, C. de P.; VIDAL, M. S.; ARAUJO, J. L. S. de; BALDANI, J. I. Identificação e quantificação de compostos indólicos no sobrenadante de Gluconacetobacter diazotrophicus, cultivada em meio liquido, por cromatografia líquida de alta eficiência (HPLC). Seropédica: Embrapa Agrobiologia, 2009. 25 p. (Embrapa Agrobiologia. Boletim de Pesquisa e Desenvolvimento, 52).| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 33 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
06/05/2008 |
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Data da última atualização: |
16/03/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
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Autoria: |
RODRIGUES, E. P.; RODRIGUES, L. da S.; OLIVEIRA, A. L. M. de; BALDANI, V. L. D.; TEIXEIRA, K. R. dos S.; URQUIAGA, S.; REIS, V. M. |
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Afiliação: |
Elisete Pains Rodrigues, UFRRJ; Luciana da Silva Rodrigues, UFRRJ; André Luiz Martinez de Oliveira, UFRRJ; Vera Lúcia Divan Baldani, Embrapa Agrobiologia; Kátia Regina dos Santos Teixeira, Embrapa Agrobiologia; Segundo Urquiaga, Embrapa Agrobiologia; Veronica Massena Reis, Embrapa Agrobiologia. |
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Título: |
Azospirillum amazonense inoculation: effects on growth, yield and N2 fixation of rice (Oryza sativa L.). |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Plant and Soil, The Netherlands, v. 302, n. 1-2, p. 249-261, jan. 2008. |
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Idioma: |
Inglês |
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Notas: |
Parceria: UFRRJ. |
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Conteúdo: |
Bacteria of the genus Azospirillum are considered to be plant-growth promoting bacteria (PGPR) and stimulate plant growth directly either by synthesising phyto-hormones or by promoting nutrition by the process of biological nitrogen fixation (BNF). Although this genus extensively studied, the effects of inoculation and the possible BNF contribution of the Azospirillum amazonense specie are very scarce. The aim of this study was to isolate, characterise and evaluate auxin production and nitrogenase activity of this species and to select, by inoculation of rice plants, promising isolates based on their ability to improve plant growth, yield and the BNF contribution. One hundred and ten isolates obtained ftom rice were characterised and grouped according to colony features. Forty-two isolates, confirmed as A. amazonense by the fluorescent in situ hybridization (FISH) technique, were tested for auxin production and nitrogenase activity in vitro. Subsequently plant growth promotion related to plant nutrition effect was evaluated, in vitro and in greenhouse experiments. The BNF contribution to plant growth was evaluated using the 15N isotope dilution technique. All A. amazonense strains tested produced indoles, but only 10% of them showed high production, above 1.33 µM mg protein-1. The nitro-genase activity also was variable and only 9% of isolates showed high nitrogenase activity and the majority (54%) exhibited a low potential. The inocu-lation of selected strains in rice under gnotobiotic conditions reduced the growth of root and aerial part when compared to the control, showing the negative effects of excess of phytohormone accumulation in the medium. However, in the greenhouse experiment, inoculation of strains of A. amazonense increased grain dry matter accumulation (7 to 11.6%), the number of panicles (3 to 18.6%) and nitrogen accumulation at grain maturation (3.5 to 18.5%). BNF contributions up to 27% were observed for A. amazonense Y2 (wild type strain). The data presented here is the first report that the PGPR effect of A. amazonense for rice plants grown under greenhouse conditions is mainly due the BNF contribution as measured by 15N isotope dilution technique, in contrast to the hormonal effect observed with other Azospirillum species studied. MenosBacteria of the genus Azospirillum are considered to be plant-growth promoting bacteria (PGPR) and stimulate plant growth directly either by synthesising phyto-hormones or by promoting nutrition by the process of biological nitrogen fixation (BNF). Although this genus extensively studied, the effects of inoculation and the possible BNF contribution of the Azospirillum amazonense specie are very scarce. The aim of this study was to isolate, characterise and evaluate auxin production and nitrogenase activity of this species and to select, by inoculation of rice plants, promising isolates based on their ability to improve plant growth, yield and the BNF contribution. One hundred and ten isolates obtained ftom rice were characterised and grouped according to colony features. Forty-two isolates, confirmed as A. amazonense by the fluorescent in situ hybridization (FISH) technique, were tested for auxin production and nitrogenase activity in vitro. Subsequently plant growth promotion related to plant nutrition effect was evaluated, in vitro and in greenhouse experiments. The BNF contribution to plant growth was evaluated using the 15N isotope dilution technique. All A. amazonense strains tested produced indoles, but only 10% of them showed high production, above 1.33 µM mg protein-1. The nitro-genase activity also was variable and only 9% of isolates showed high nitrogenase activity and the majority (54%) exhibited a low potential. The inocu-lation of selected strains in rice under... Mostrar Tudo |
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Palavras-Chave: |
Biological nitrogen fixation; BNF; FBN; Fixação biológica de nitrogênio; Growth. |
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Thesagro: |
Arroz; Crescimento; Oryza Sativa. |
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Thesaurus NAL: |
Azospirillum amazonense; rice. |
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Categoria do assunto: |
-- |
|
Marc: |
LEADER 03231naa a2200325 a 4500
001 1629942
005 2015-03-16
008 2008 bl --- 0-- u #d
100 1 $aRODRIGUES, E. P.
245 $aAzospirillum amazonense inoculation$beffects on growth, yield and N2 fixation of rice (Oryza sativa L.).
260 $c2008
500 $aParceria: UFRRJ.
520 $aBacteria of the genus Azospirillum are considered to be plant-growth promoting bacteria (PGPR) and stimulate plant growth directly either by synthesising phyto-hormones or by promoting nutrition by the process of biological nitrogen fixation (BNF). Although this genus extensively studied, the effects of inoculation and the possible BNF contribution of the Azospirillum amazonense specie are very scarce. The aim of this study was to isolate, characterise and evaluate auxin production and nitrogenase activity of this species and to select, by inoculation of rice plants, promising isolates based on their ability to improve plant growth, yield and the BNF contribution. One hundred and ten isolates obtained ftom rice were characterised and grouped according to colony features. Forty-two isolates, confirmed as A. amazonense by the fluorescent in situ hybridization (FISH) technique, were tested for auxin production and nitrogenase activity in vitro. Subsequently plant growth promotion related to plant nutrition effect was evaluated, in vitro and in greenhouse experiments. The BNF contribution to plant growth was evaluated using the 15N isotope dilution technique. All A. amazonense strains tested produced indoles, but only 10% of them showed high production, above 1.33 µM mg protein-1. The nitro-genase activity also was variable and only 9% of isolates showed high nitrogenase activity and the majority (54%) exhibited a low potential. The inocu-lation of selected strains in rice under gnotobiotic conditions reduced the growth of root and aerial part when compared to the control, showing the negative effects of excess of phytohormone accumulation in the medium. However, in the greenhouse experiment, inoculation of strains of A. amazonense increased grain dry matter accumulation (7 to 11.6%), the number of panicles (3 to 18.6%) and nitrogen accumulation at grain maturation (3.5 to 18.5%). BNF contributions up to 27% were observed for A. amazonense Y2 (wild type strain). The data presented here is the first report that the PGPR effect of A. amazonense for rice plants grown under greenhouse conditions is mainly due the BNF contribution as measured by 15N isotope dilution technique, in contrast to the hormonal effect observed with other Azospirillum species studied.
650 $aAzospirillum amazonense
650 $arice
650 $aArroz
650 $aCrescimento
650 $aOryza Sativa
653 $aBiological nitrogen fixation
653 $aBNF
653 $aFBN
653 $aFixação biológica de nitrogênio
653 $aGrowth
700 1 $aRODRIGUES, L. da S.
700 1 $aOLIVEIRA, A. L. M. de
700 1 $aBALDANI, V. L. D.
700 1 $aTEIXEIRA, K. R. dos S.
700 1 $aURQUIAGA, S.
700 1 $aREIS, V. M.
773 $tPlant and Soil, The Netherlands$gv. 302, n. 1-2, p. 249-261, jan. 2008.
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